Journal: International Journal of Molecular Sciences

Article Title: The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia

doi: 10.3390/ijms22168499

Figure Lengend Snippet: Levels of NET and TrkC in T cells, and NT-3 in plasma. Legend: quantification of NET and TrkC levels in T cells and NT-3 in plasma, and their respective scatter plots and bar graphs (mean ± SD). ( a ) NEText (45 patients with schizophrenia vs. 31 controls) and TrkC (31 patients with schizophrenia vs. 22 controls) levels were measured by Western blot and normalized with the housekeeping protein β-actin. Relative units refer to the mean of control values after normalization. The results showed a reduction in NET levels ( p < 0.0001) and TrkC levels ( p = 0.0032) in T cells of patients with schizophrenia compared to the control group. ( b ) The analysis of NT-3 (54 patients with schizophrenia and 54 healthy controls) in plasma revealed a reduction in this neurotrophin in the plasma of patients in comparison with controls ( p = 0.0040), whose concentration was measured by ELISA using a polynomial regression method (Y = absorbance; X = concentration, degree = 3; R 2 = 0.9999).

Article Snippet: The membranes were then incubated overnight at 4 °C over stirrers with the primary antibody: anti-NET extracellular rabbit polyclonal antibody 1:1000 (AMT-002, Alomone Labs, Jerusalem, Israel), TrkC rabbit polyclonal antibody 1:1000 (sab1306628, Sigma-Aldrich, St. Louis, MO, USA), anti-NT3 rabbit polyclonal antibody 1:1000 (WB) (ANT-003, Alomone Labs, Jerusalem, Israel), anti-phosphotyrosine (p-Tyr) mouse monoclonal antibody 1:1000 (ab10321, Abcam, Cambridge, UK), or anti-β-actin rabbit polyclonal antibody 1:14,000 (600-401-886, Rockland Immunochemicals, Limerick, PA, USA).

Techniques: Clinical Proteomics, Western Blot, Control, Comparison, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Syringin and Phillygenin—Natural Compounds with a Potential Role in Preventing Lipid Deposition in Macrophages in the Context of Human Atherosclerotic Plaque

doi: 10.3390/ijms26136444

Figure Lengend Snippet: ( A ) The effect of syringin (Syr) and phillygenin (Phil) on ABCA1 expression. Quantification of protein ABCA1 expression was performed by western blot in cholesterol-induced macrophages. The results were quantified by densitometry ( n = 3). β-actin was used as an internal control. Statistical significance ** p < 0.001 compared to Chol20 (#). ( B ) The intracellular secretion of HO-1 was analyzed by an ELISA test. The results are presented as pg/g of cellular protein ± SEM ( n = 3). Statistical significance ** p < 0.001 compared to cholesterol-induced macrophages (#). ( C ) The protein expression of Nrf2 was analyzed by western blot in macrophages. The results were quantified by densitometry. β-actin was used as an internal control. Data are presented as the mean ± SEM for each group ( n = 3). Statistical significance * p < 0.05, ** p < 0.001 compared to Chol20 (#).

Article Snippet: Rabbit anti-β-actin polyclonal antibody was from FineTest (Wuhan, China).

Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay